Lamb1^em1Nki
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lamb1^em1Nki
• Name: laminin B1; endonuclease-mediated mutation 1, The Netherlands Cancer Institute
• MGI ID: MGI:6274681
• Synonyms: LamB1Dendra2
• Gene: Lamb1 Location: Chr12:31315233-31379643 bp, + strand Genetic Position: Chr12, 13.39 cM
• Alliance: Lamb1^em1Nki page

Mutation origin

• Strain of Origin: FVB/NRj

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR-targeting introduced an in-frame knock-in containing a flexible linker, the Dendra2 reporter gene and 3xFlag.STOP in the last exon at the stop codon using guide RNA(5'-TTACAGCACCTGCTTATAACAGG-3'). (J:334553)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lamb1 Mutation: 86 strains or lines available

References

• Original: J:334553 Morgner J, et al., A Lamb1Dendra2 mouse model identifies basement-membrane-producing origins and dynamics in PyMT breast tumors. Dev Cell. 2023 Apr 10;58(7):535-549.e5
• All: 1 reference(s)