Rnpc3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnpc3^{tm1c(EUCOMM)Wtsi}
• Name: RNA-binding region (RNP1, RRM) containing 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6269405
• Synonyms: Rnpc3^lox
• Gene: Rnpc3 Location: Chr3:113398716-113423798 bp, - strand Genetic Position: Chr3, 49.35 cM, cytoband G1
• Alliance: Rnpc3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Rnpc3 gene page

Mutation origin

• Mutant Cell Line: EPD0441_1_B10
• Germline Transmission: Earliest citation of germline transmission: J:267519
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 113415224 of Chromosome 3 upstream of the critical exons 4 and 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 113416571. Exons 4 and 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exons 4 and 5 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267519)

Expression

In Structures Affected by this Mutation:

15 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Rnpc3^{tm1c(EUCOMM)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnpc3 Mutation: 41 strains or lines available

References

• Original: J:267519 Doggett K, et al., Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice. RNA. 2018 Dec;24(12):1856-1870
• All: 2 reference(s)