Washc5^tm1.2Cbee
Targeted Allele Detail

Summary

• Symbol: Washc5^tm1.2Cbee
• Name: WASH complex subunit 5; targeted mutation 1.2, Christian Beetz
• MGI ID: MGI:6256428
• Synonyms: E430025E21Rik^ko
• Gene: Washc5 Location: Chr15:59203846-59246016 bp, - strand Genetic Position: Chr15, 25.17 cM
• Alliance: Washc5^tm1.2Cbee page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266630
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted 216 bp downstream of exon 12 and a PGK promoter-driven neomycin resistance cassette flanked by frt-sites and preceded by another loxP site were inserted 265 bp upstream of exon 12. The selection cassette was removed via Flp-mediated recombination. Cre-mediated recombination removed exon 12. (J:266630)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Washc5 Mutation: 56 strains or lines available

References

• Original: J:266630 Jahic A, et al., The spectrum of KIAA0196 variants, and characterization of a murine knockout: implications for the mutational mechanism in hereditary spastic paraplegia type SPG8. Orphanet J Rare Dis. 2015 Nov 16;10:147
• All: 1 reference(s)