Col1a1^{tm1(tetO-GFP/RNAi:Col1a1)Schup}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-GFP/RNAi:Col1a1)Schup}
• Name: collagen, type I, alpha 1; targeted mutation 1, Detlef Schuppan
• MGI ID: MGI:6201737
• Synonyms: Tet-GFP-shRNA-Col1a1-7
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-GFP/RNAi:Col1a1)Schup} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265449
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-GFP/RNAi:Col1a1)Schup} involves 1 genes/genome features (Col1a1) View all
• Mutation details: Flp-mediated recombination introduced a splice acceptor-polyA sequence, a TRE sequence, a GFP gene, an shRNA targeting Col1a1, a poly A sequence, and a PGK promoter into the FRT flanked homing site in the 3' UTR. The shRNA in this construct had the lowest predicted off-target effect of those tested. (J:265449)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae}.

References

• Original: J:265449 Molokanova O, et al., Inducible knockdown of procollagen I protects mice from liver fibrosis and leads to dysregulated matrix genes and attenuated inflammation. Matrix Biol. 2018 Mar;66:34-49
• All: 1 reference(s)