Vps35^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Vps35^{tm1c(EUCOMM)Hmgu}
• Name: VPS35 retromer complex component; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6197919
• Synonyms: Vps35^f
• Gene: Vps35 Location: Chr8:85987014-86026146 bp, - strand Genetic Position: Chr8, 41.61 cM
• Alliance: Vps35^{tm1c(EUCOMM)Hmgu} page
• IMPC: Vps35 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264396
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 86009499 of Chromosome 8 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 86011277. The critical exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. Subsequent cre expression results in a knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:264396)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vps35 Mutation: 46 strains or lines available

References

• Original: J:264396 Tang FL, et al., VPS35 Deficiency or Mutation Causes Dopaminergic Neuronal Loss by Impairing Mitochondrial Fusion and Function. Cell Rep. 2015 Sep 8;12(10):1631-43
• All: 10 reference(s)