Gsdmd^em4Fcw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gsdmd^em4Fcw
• Name: gasdermin D; endonuclease-mediated mutation 4, Fengchao Wang
• MGI ID: MGI:6196334
• Gene: Gsdmd Location: Chr15:75734176-75739257 bp, + strand Genetic Position: Chr15, 35.0 cM, cytoband D3-E1
• Alliance: Gsdmd^em4Fcw page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cas9 mRNA and gRNA (5'-AGCATCCTGGCATTCCGAG-3') were co-injected into C57BL/6NCrl zygotes. Founders with frameshift mutations were screened via T7E1 assay and validated by DNA sequencing. This mutation carries an 37 bp deletion in exon 5 of the gene. (J:226781)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gsdmd Mutation: 32 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 12 reference(s)