Tmem119^{em1(cre/ERT2)Gfng}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmem119^{em1(cre/ERT2)Gfng}
• Name: transmembrane protein 119; endonuclease-mediated mutation 1, Guoping Feng
• MGI ID: MGI:6195002
• Synonyms: Tmem119-CreERT2
• Gene: Tmem119 Location: Chr5:113931790-113938530 bp, - strand Genetic Position: Chr5, 55.55 cM
• Alliance: Tmem119^{em1(cre/ERT2)Gfng} page

Mutation origin

• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tmem119^{em1(cre/ERT2)Gfng} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tmem119 (MGI:2385228)

• Mutation details: The allele was created using CRISPR/cas9 endonuclease-mediated genome editing to target the stop codon of the transmembrane protein 119 locus (Tmem119) on chromosome 5. The cas9-RNP complex and a 2A-CreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the CreER fusion gene with ~1.5 kbp homology arms were injected into the pronucleus of C57BL/6NTac mouse embryos. Founders were bred to C57BL/6J mice for germline transmission. (J:280123)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tmem119 (mouse) Summary of all recombinase alleles driven by Tmem119. Comparative matrix view of recombinase activities Alliance mouse Tmem119 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tmem119 Mutation: 25 strains or lines available

References

• Original: J:280123 Kaiser T, et al., Tmem119-EGFP and Tmem119-CreERT2 Transgenic Mice for Labeling and Manipulating Microglia. eNeuro. 2019 Jul/Aug;6(4):ENEURO.0448-18.2019
• All: 18 reference(s)