Tyr^em2Kest
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tyr^em2Kest
• Name: tyrosinase; endonuclease-mediated mutation 2, Robert A Kesterson
• MGI ID: MGI:6159170
• Synonyms: chandana
• Gene: Tyr Location: Chr7:87073979-87142637 bp, - strand Genetic Position: Chr7, 49.01 cM
• Alliance: Tyr^em2Kest page

Mutation origin

• Strain of Origin: (B6(Cg)-Tyr^c-2J x C57BL/6J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Hypomorph)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mediated mutagenesis created a 15 bp in-frame deletion in a region proximal to the 5' end of exon 1 resulting in a 5 amino acid deletion (GPQFPFKGVDDRESW to GPQLDDRESW) and a 3 bp in-frame deletion resulting in a loss of threonine at position 244. (J:248812)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tyr Mutation: 382 strains or lines available

References

• Original: J:248812 Challa AK, et al., Novel Hypomorphic Alleles of the Mouse Tyrosinase Gene Induced by CRISPR-Cas9 Nucleases Cause Non-Albino Pigmentation Phenotypes. PLoS One. 2016;11(5):e0155812
• All: 1 reference(s)