Top2a^em2(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Top2a^em2(IMPC)Tcp
• Name: topoisomerase (DNA) II alpha; endonuclease-mediated mutation 2, The Centre for Phenogenomics
• MGI ID: MGI:6157267
• Gene: Top2a Location: Chr11:98883769-98915015 bp, - strand Genetic Position: Chr11, 62.91 cM
• Alliance: Top2a^em2(IMPC)Tcp page
• IMPC: Top2a gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR0590 was generated at The Centre for Phenogenomics by injecting Cas9 mRNA and four guide RNAs with spacer sequences of GCCCTGCATAAGAACATGGG and GTCAGCATGTATTTTGGGTC targeting the 5' side and TTTGAGTTAGAGTGCAGTAT and ATGCCTGTGCCTGATCTGAC targeting the 3' side of exons ENSMUSE00000648121 (exon 6), ENSMUSE00000648120 (exon 7), ENSMUSE00000648119 (exon 8), ENSMUSE00000648118 (exon 9), and ENSMUSE00000648117 (exon 10). This resulted in a 2,899-bp deletion of Chr11 from 99014059 to 99016957_ins.GATA and a 9-bp deletion of Chr11 from 99013968 to 99013976 (GRCm38). (J:237616)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Top2a Mutation: 87 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 2 reference(s)