Tg(CAG-Ptp4a2,-EGFP)11Zyz
Transgene Detail

Summary

• Symbol: Tg(CAG-Ptp4a2,-EGFP)11Zyz
• Name: transgene insertion 11, Zhong-Yin Zhang
• MGI ID: MGI:6153153
• Synonyms: PRL2^Testis
• Transgene: Tg(CAG-Ptp4a2,-EGFP)11Zyz Location: unknown
• Alliance: Tg(CAG-Ptp4a2,-EGFP)11Zyz page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-Ptp4a2,-EGFP)11Zyz expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ptp4a2 (MGI:1277117)	derived from MGI:6153150 allele after cre-mediated recombination

• Mutation details: This allele is derived from the Tg(CAG-lacZ,-Ptp4a2,-EGFP)11Zyz allele. Using the piZEG plasmid, a transgene construct comprising the following elements was inserted into the genome: a CAG promoter, a lacZ enzymatic reporter gene cassette with triple poly(A) signals flanked by loxP sites, the coding region from the cDNA of the murine Ptp4a2 gene, an IRES, the EGFP fluorescent reporter gene and a poly(A) signal. The lacZ cassette was subsequently removed bthrough cre-medoated recombination y crossing with the Tg(EIIa-cre)C5379Lmgd cre-expressing strain. The CAG promoter now drives the expression of the inserted Ptp4a2 gene and the EGFP reporter gene. (J:250112)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:250112 Bai Y, et al., Role of phosphatase of regenerating liver 1 (PRL1) in spermatogenesis. Sci Rep. 2016 Sep 26;6:34211
• All: 1 reference(s)