Pgp^tm1.2Ango
Targeted Allele Detail

Summary

• Symbol: Pgp^tm1.2Ango
• Name: phosphoglycolate phosphatase; targeted mutation 1.2, Antje Gohla
• MGI ID: MGI:6150833
• Synonyms: Pgp^D34N
• Gene: Pgp Location: Chr17:24689400-24692084 bp, + strand Genetic Position: Chr17, 12.39 cM, cytoband A3.3
• Alliance: Pgp^tm1.2Ango page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259970
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site downstream of exon 2. Also inserted downstream of exon 2 was a cDNA for the endogenous gene where a point mutation was engineered to change aspartate codon 34 into an asparagine codon (p.D34N or p.Asp34Asn). The neo cassette was removed through subsequent flp-mediated recombination and exons 1-2 (comprising the entire gene) were deleted through subsequent cre-mediated recombination. This leaves the mutated cDNA which codes for a kinase-dead version of the protein. (J:259970)

Expression

In Mice Carrying this Mutation:	20 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pgp Mutation: 6 strains or lines available

References

• Original: J:259970 Segerer G, et al., An essential developmental function for murine phosphoglycolate phosphatase in safeguarding cell proliferation. Sci Rep. 2016 Oct 12;6:35160
• All: 1 reference(s)