Rps14^tm1.1Ble
Targeted Allele Detail

Summary

• Symbol: Rps14^tm1.1Ble
• Name: ribosomal protein S14; targeted mutation 1.1, Benjamin L Ebert
• MGI ID: MGI:6147818
• Synonyms: Rps14^fl
• Gene: Rps14 Location: Chr18:60907668-60911618 bp, + strand Genetic Position: Chr18, 34.4 cM
• Alliance: Rps14^tm1.1Ble page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233322
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site, containing engineered ApaLI and Bcl sites, was inserted 379 bp upstream of exon 2. A FRT site, loxP site, neomycin selection cassette, FRT site, and loxP site were inserted 255 bp downstream of exon 4. Flp-mediated recombination removed the selection cassette, leaving exons 2-4 floxed. (J:233322)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rps14 Mutation: 18 strains or lines available

References

• Original: J:233322 Schneider RK, et al., Rps14 haploinsufficiency causes a block in erythroid differentiation mediated by S100A8 and S100A9. Nat Med. 2016 Mar;22(3):288-97
• All: 4 reference(s)