Gt(ROSA)26Sor^{em1(CAG-TMEM14B,-EGFP)Xqw}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-TMEM14B,-EGFP)Xqw}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Xiaoqun Wang
• MGI ID: MGI:6121115
• Synonyms: TMEM14B^flox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-TMEM14B,-EGFP)Xqw} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A vector containing the following elements was targeted to the host locus: the CAG enhancer/promoter, a loxP site flanked STOP cassette, three copies of the sequence coding for the FLAG tag, the cDNA for the coding sequence of the human TMEM14B gene, an IRES, the EGFP fluorescent reporter gene, WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) sequence and poly(A) sequence. Using Crispr/CAS9 technology, the vector plus Cas9, sgRNAs and template DNA were injected into C57BL/6 zygotes. This creates a conditional-ready allele that will express the human gene and the reporter gene only after cre-mediated excision of the STOP cassette. The human gene does not have a mouse ortholog, as it is a family member unique to primates. (J:253976)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:253976 Liu J, et al., The Primate-Specific Gene TMEM14B Marks Outer Radial Glia Cells and Promotes Cortical Expansion and Folding. Cell Stem Cell. 2017 Nov 2;21(5):635-649.e8
• All: 1 reference(s)