Tmem192^{tm2d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tmem192^{tm2d(EUCOMM)Hmgu}
• Name: transmembrane protein 192; targeted mutation 2d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6115602
• Synonyms: tm1d, TMEM192^-
• Gene: Tmem192 Location: Chr8:65399837-65421685 bp, + strand Genetic Position: Chr8, 32.44 cM, cytoband B3.3
• Alliance: Tmem192^{tm2d(EUCOMM)Hmgu} page
• IMPC: Tmem192 gene page

Mutation origin

• Mutant Cell Line: HEPD0785_6_G07
• Germline Transmission: Earliest citation of germline transmission: J:256294
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 65411506 of Chromosome 8 upstream of the critical exon (exon 3) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 65412540. Exon 3 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon. Western blot analysis of total organ lysates confirmed the absence of expression of both the monomeric full-length protein and the N-terminal fragment derived by proteolysis from homozygous mutant mice. (J:256294)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem192 Mutation: 14 strains or lines available

References

• Original: J:256294 Nguyen TL, et al., Functional characterization of the lysosomal membrane protein TMEM192 in mice. Oncotarget. 2017 Jul 4;8(27):43635-43652
• All: 1 reference(s)