Dennd1b^tm1.2Achn
Targeted Allele Detail

Summary

• Symbol: Dennd1b^tm1.2Achn
• Name: DENN domain containing 1B; targeted mutation 1.2, Andrew C Chan
• MGI ID: MGI:6113476
• Gene: Dennd1b Location: Chr1:138891447-139103781 bp, + strand Genetic Position: Chr1, 60.84 cM
• Alliance: Dennd1b^tm1.2Achn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229307
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Recombination of loxP sites that flanked exon 6 resulted in the deletion of these sequences in the germline. Deletion of exon 6 results in a loss of function of the gene by generating a frameshift from exon 5 to exons 7 - 10 with the introduction of a premature stop codon in exon 7. Western blot and qRT-PCR experiments confirmed the absence of expression from this allele. (J:229307)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dennd1b Mutation: 65 strains or lines available

References

• Original: J:229307 Yang CW, et al., Regulation of T Cell Receptor Signaling by DENND1B in TH2 Cells and Allergic Disease. Cell. 2016 Jan 14;164(1-2):141-55
• All: 1 reference(s)