Ndst4^tm1.1Ntuy
Targeted Allele Detail

Summary

• Symbol: Ndst4^tm1.1Ntuy
• Name: N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4; targeted mutation 1.1, Ya-Chien Yang
• MGI ID: MGI:6111332
• Synonyms: Ndst4^-
• Gene: Ndst4 Location: Chr3:125197725-125522548 bp, + strand Genetic Position: Chr3, 54.68 cM, cytoband H1
• Alliance: Ndst4^tm1.1Ntuy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252046
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites were inserted upstream of exon 2 and downstream of exon 14 through homologous recombination. Cre-mediated recombination in ES cells resulted in the deletion of exons 2-14. The deletion resulted in the elimination of the N-deacetylase and N-sulfotransferase activities of the protein. RT-PCR analysis of cerebral RNA confirmed the absence of mRNA transcripts in homozygous mutant mice. (J:252046)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ndst4 Mutation: 63 strains or lines available

References

• Original: J:252046 Jao TM, et al., Alteration of colonic epithelial cell differentiation in mice deficient for glucosaminyl N-deacetylase/N-sulfotransferase 4. Oncotarget. 2016 Dec 20;7(51):84938-84950
• All: 1 reference(s)