Rufy3^tm1.1Migar
Targeted Allele Detail

Summary

• Symbol: Rufy3^tm1.1Migar
• Name: RUN and FYVE domain containing 3; targeted mutation 1.1, Michihirio Igarashi
• MGI ID: MGI:6111136
• Gene: Rufy3 Location: Chr5:88712899-88799251 bp, + strand Genetic Position: Chr5, 43.77 cM
• Alliance: Rufy3^tm1.1Migar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:253649
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An FRT site flanked pgk-Neo cassette and a loxP sequence were inserted 249 bp upstream of exon 3, and a second loxP sequence 269 bp downstream of exon 3. Exon 3 was subsequently deleted via Cre-mediated recombination. Western blot analysis showed that no detectable encoded protein was expressed from this allele. (J:253649)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rufy3 Mutation: 34 strains or lines available

References

• Original: J:253649 Honda A, et al., Rufy3 is an adapter protein for small GTPases that activates a Rac guanine nucleotide exchange factor to control neuronal polarity. J Biol Chem. 2017 Dec 22;292(51):20936-20946
• All: 1 reference(s)