Prdx6^tm5.1Abf
Targeted Allele Detail

Summary

• Symbol: Prdx6^tm5.1Abf
• Name: peroxiredoxin 6; targeted mutation 5.1, Aron B Fisher
• MGI ID: MGI:5911855
• Synonyms: D140A
• Gene: Prdx6 Location: Chr1:161067682-161078780 bp, - strand Genetic Position: Chr1, 69.75 cM
• Alliance: Prdx6^tm5.1Abf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239138
• Parent Cell Line: EAP6 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Single point mutation
• Mutation details: The targeting vector is designed to insert, by site-directed mutagenesis, a point mutation (GAC to GCC) at nucleotide 419 followed by an FRT-flanked neomycin cassette downstream of exon 4. The mutation results in an alteration of the corresponding amino acid from aspartic acid to alanine (D140A) at codon 140. Flp-mediated recombination removed the FRT-flanked neo cassette. The mutation does not affect peroxidase activity, but does eliminate phospholipase A2 activity. (J:239138)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prdx6 Mutation: 52 strains or lines available

References

• Original: J:239138 Li H, et al., Critical role of peroxiredoxin 6 in the repair of peroxidized cell membranes following oxidative stress. Free Radic Biol Med. 2015 Oct;87:356-65
• All: 3 reference(s)