Rr492em1.1Chalu
Endonuclease-mediated Allele Detail
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Symbol: |
Rr492em1.1Chalu |
Name: |
regulatory region 492; endonuclease-mediated mutation 1.1, James Noonan |
MGI ID: |
MGI:5911848 |
Synonyms: |
PDE-, Pitx1em1.1Chalu |
Gene: |
Rr492 Location: Chr13:56108910-56111397 bp Genetic Position: Chr13, Syntenic
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Alliance: |
Rr492em1.1Chalu page
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Allele Type: |
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Endonuclease-mediated (Hypomorph) |
Mutation: |
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Intragenic deletion
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Mutation details: CRISPR/Cas technology was employed to introduce loxP sequences flanking a 13-kb enhancer residing 132 kb upstream of the promoter (Pitx distal enhancer or PDE), shown via chromatin conformation capture (3C) and chromosome conformation capture-on-chip (4C) analyses to interact topologically with the target gene in hind limbs The floxed sequence was deleted by Cre recombinase. The deleted sequence spans chr13:56102380-56115399 (GRCm39). In heterozygous mutant embryos at E11.5, Pitx1 expression is reduced by 15% in the hindlimb and by 39% in the mandibular arch.
(J:94077, J:264122)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Rr492 Mutation: |
0 strains or lines available
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Original: |
J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015; |
All: |
2 reference(s) |
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