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Rr492em1.1Chalu
Endonuclease-mediated Allele Detail
Summary
Symbol: Rr492em1.1Chalu
Name: regulatory region 492; endonuclease-mediated mutation 1.1, James Noonan
MGI ID: MGI:5911848
Synonyms: PDE-, Pitx1em1.1Chalu
Gene: Rr492  Location: Chr13:56108910-56111397 bp  Genetic Position: Chr13, Syntenic
Alliance: Rr492em1.1Chalu page
Mutation
origin
Strain of Origin:  C57BL/6J
Mutation
description
Allele Type:    Endonuclease-mediated (Hypomorph)
Mutation:    Intragenic deletion
 
Mutation detailsCRISPR/Cas technology was employed to introduce loxP sequences flanking a 13-kb enhancer residing 132 kb upstream of the promoter (Pitx distal enhancer or PDE), shown via chromatin conformation capture (3C) and chromosome conformation capture-on-chip (4C) analyses to interact topologically with the target gene in hind limbs The floxed sequence was deleted by Cre recombinase. The deleted sequence spans chr13:56102380-56115399 (GRCm39). In heterozygous mutant embryos at E11.5, Pitx1 expression is reduced by 15% in the hindlimb and by 39% in the mandibular arch. (J:94077, J:264122)
Expression
In Mice Carrying this Mutation: 27 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rr492 Mutation:  0 strains or lines available
References
Original:  J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory