Rr492^em1.1Chalu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr492^em1.1Chalu
• Name: regulatory region 492; endonuclease-mediated mutation 1.1, James Noonan
• MGI ID: MGI:5911848
• Synonyms: PDE-, Pitx1^em1.1Chalu
• Gene: Rr492 Location: Chr13:56108910-56111397 bp Genetic Position: Chr13, Syntenic
• Alliance: Rr492^em1.1Chalu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Hypomorph)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas technology was employed to introduce loxP sequences flanking a 13-kb enhancer residing 132 kb upstream of the promoter (Pitx distal enhancer or PDE), shown via chromatin conformation capture (3C) and chromosome conformation capture-on-chip (4C) analyses to interact topologically with the target gene in hind limbs The floxed sequence was deleted by Cre recombinase. The deleted sequence spans chr13:56102380-56115399 (GRCm39). In heterozygous mutant embryos at E11.5, Pitx1 expression is reduced by 15% in the hindlimb and by 39% in the mandibular arch. (J:94077, J:264122)

Expression

In Mice Carrying this Mutation:

27 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr492 Mutation: 0 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 2 reference(s)