Pitx1^em1.1Chalu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pitx1^em1.1Chalu
• Name: paired-like homeodomain transcription factor 1; endonuclease-mediated mutation 1.1, James Noonan
• MGI ID: MGI:5911848
• Synonyms: PDE-
• Gene: Pitx1 Location: Chr13:55972864-55984005 bp, - strand Genetic Position: Chr13, 30.06 cM
• Alliance: Pitx1^em1.1Chalu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Hypomorph)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas technology was employed to introduce loxP sequences flanking a 13-kb enhancer residing 132 kb 3' of the promoter (Pitx distal enhancer or PDE), shown via chromatin conformation capture (3C) and chromosome conformation capture-on-chip (4C) analyses to interact topologically with the target gene. The floxed sequence was deleted by Cre recombinase. The deleted sequence spans Chr 13 nucleotides 56,055,928 through 56,068,947. In heterozygous mutant embryos at E11.5, Pitx1 expression is reduced by 15% in the hindlimb and by 39% in the mandibular arch. (J:94077, J:264122)

Expression

In Mice Carrying this Mutation:

27 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pitx1 Mutation: 10 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 2 reference(s)