Tn(pb-EGFP/Tdrd6)#Jess
Transgene Detail

Summary

• Symbol: Tn(pb-EGFP/Tdrd6)#Jess
• Name: transposon insertion, Rolf Jessberger
• MGI ID: MGI:5910794
• Synonyms: Tdrd6-LAP
• Transgene: Tn(pb-EGFP/Tdrd6)#Jess Location: unknown
• Alliance: Tn(pb-EGFP/Tdrd6)#Jess page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Transposon induced (Inserted expressed sequence, No functional change, Reporter)
• Mutation: Transposon insertion
• Tn(pb-EGFP/Tdrd6)#Jess expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tdrd6 (MGI:2679727)

• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted into an artificial intron (flanked by splice donor and acceptor sites) inserted into the EGFP (enhanced green fluorescent protein) gene. This construct, followed by PreScission cleavage site, S-peptide and TEV cleavage site sequences, was then inserted upstream of the ATG translation start site of the mouse gene in BAC clone RP23-283P8. A cassette carrying the piggyBac inverted repeats was inserted into the vector. To integrate the transgenic construct into the genome, the vector was injected into the pronucleus of fertilized C57B6L/6 eggs together with piggyBac transposase mRNA. This creates an allele for a Localization and Affinity Purification (LAP)-tagged protein, with the N-terminally attached EGFP serving as a tag both in localization studies and in immunoprecipitation experiments. Immunoblots showed protein expression from this allele in testes. Subsequent crossing with mice carying the Tdrd6^tm1Jess allele showed that the transgene was able to rescue that null allele. A number of founders were established but no line information is specified (indicated by the pound # sign). (J:242066)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:242066 Akpinar M, et al., TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes. PLoS Genet. 2017 Mar;13(3):e1006660
• All: 1 reference(s)