Gnrhr^{tm2.1(GNRHR)Djb}
Targeted Allele Detail

Summary

• Symbol: Gnrhr^{tm2.1(GNRHR)Djb}
• Name: gonadotropin releasing hormone receptor; targeted mutation 2.1, Daniel J Bernard
• MGI ID: MGI:5908153
• Synonyms: Gnrhr^Ctail
• Gene: Gnrhr Location: Chr5:86328613-86345760 bp, - strand Genetic Position: Chr5, 43.56 cM
• Alliance: Gnrhr^{tm2.1(GNRHR)Djb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324870
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Gnrhr^{tm2.1(GNRHR)Djb} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GNRHR (2798)

  Gnrhr

  (MGI:95790)

• Mutation details: A loxP-flanked exon 3 with a polyA sequence, FRT-flanked neomycin selection cassette, and a modified exon 3 encoding the C-terminus of mouse Gnrhr fused in-frame with the intracellular cytosolic tail (Ctail) of the chicken GNRHR replaced the endogenous exon 3. The neomycin selection cassette was removed via flp-mediated recombination. Cre-mediated recombination removed the floxed exon 3 leaving an exon 3 composed of the mouse C-terminus fused with the chicken intracellular cytosolic trail. (J:324870)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnrhr Mutation: 20 strains or lines available

References

• Original: J:324870 Toufaily C, et al., Addition of a carboxy-terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice. Elife. 2021 Dec 23;10:e72937
• All: 1 reference(s)