Prdx6^tm4Abf
Targeted Allele Detail

Summary

• Symbol: Prdx6^tm4Abf
• Name: peroxiredoxin 6; targeted mutation 4, Aron B Fisher
• MGI ID: MGI:5908011
• Synonyms: H26A Prdx6, H26A Prdx6 knock-in, Prdx6 H26A
• Gene: Prdx6 Location: Chr1:161067682-161078780 bp, - strand Genetic Position: Chr1, 69.75 cM
• Alliance: Prdx6^tm4Abf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231458
• Parent Cell Line: EAP6 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The mutant allele contains point mutations changing the nucleotides corresponding to positions 76 and 77 of the mRNA sequence (NM_007453.4) from CA to GC, replacing the CAC histidine codon at amino acid position 26 with GCC, an alanine codon (H26A). This histidine, with S32 and D140, constitute the phospholipase A2 (PLA2) catalytic site. In addition, a frt site-flanked neomycin resistance ("pgk EM7 neo polyA") cassette has been introduced between exons 1 and 2. The H26A mutant protein has lost phospholipase A 2 (PLA2) activity, but retains lysophosphatidylcholine acyl transferase (LPCAT) activity; as for peroxidase activity, it retains the ability to reduce short chain hydroperoxides, but cannot reduce phospholipid hydroperoxides. (J:231458)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdx6 Mutation: 52 strains or lines available

References

• Original: J:231458 Fisher AB, et al., A novel lysophosphatidylcholine acyl transferase activity is expressed by peroxiredoxin 6. J Lipid Res. 2016 Apr;57(4):587-96
• All: 1 reference(s)