Uhrf2^em1Yoz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Uhrf2^em1Yoz
• Name: ubiquitin-like, containing PHD and RING finger domains 2; endonuclease-mediated mutation 1, Yongxiang Zhao
• MGI ID: MGI:5907901
• Gene: Uhrf2 Location: Chr19:30007920-30071126 bp, + strand Genetic Position: Chr19, 24.73 cM
• Alliance: Uhrf2^em1Yoz page

Mutation origin

• Strain of Origin: FVB/N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Two sgRNAs (5'-GGTCCGCACCATCGACGGCTCC-3' and 5'-GGAGCTGCGCGAGCGGGTCT-3') were used to target the start of the coding region of exon 1 with CRISPR/Cas9. A number of different deletions were found, with this allele, number 10, representing a 277 bp deletion removing part of the 5' UTR and the start of the coding region (-180 to +97). The absence of transcripts from this allele was confirmed by qPCR and the absence of peptides by Western blot analysis of thymus and brain samples. (J:241384, J:243187)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Uhrf2 Mutation: 36 strains or lines available

References

• Original: J:243187 Li D, et al., Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):681-3
• All: 3 reference(s)