P3h4^tm1.2Rmr
Targeted Allele Detail

Summary

• Symbol: P3h4^tm1.2Rmr
• Name: prolyl 3-hydroxylase family member 4 (non-enzymatic); targeted mutation 1.2, Roy Morello
• MGI ID: MGI:5905852
• Gene: P3h4 Location: Chr11:100299293-100305542 bp, - strand Genetic Position: Chr11, 63.47 cM
• Alliance: P3h4^tm1.2Rmr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232567
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J x 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 7. An additional loxP site was inserted downstream of exon 8. Flp-mediated recombination removed the selection cassette and left exons 7 and 8 floxed. Cre-mediated recombination removed exons 7 and 8. Western blot analysis confirmed the absence of protein expression in calvaria, tail, kidney and rib. (J:232567)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P3h4 Mutation: 10 strains or lines available

References

• Original: J:232567 Heard ME, et al., Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation. PLoS Genet. 2016 Apr;12(4):e1006002
• All: 2 reference(s)