Map3k14^tm1.2Gne
Targeted Allele Detail

Summary

• Symbol: Map3k14^tm1.2Gne
• Name: mitogen-activated protein kinase kinase kinase 14; targeted mutation 1.2, Genentech
• MGI ID: MGI:5903085
• Synonyms: NIK-KO
• Gene: Map3k14 Location: Chr11:103110590-103158227 bp, - strand Genetic Position: Chr11, 66.8 cM
• Alliance: Map3k14^tm1.2Gne page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240453
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted into intron 1 and a further loxP site into intron 2. The neo cassette was deleted through subsequent cre-mediated recombination, leaving a conditional-ready allele with exon 2 floxed. A constitutive null allele was then created through cross-breeding with a cre allele containing strain. Absence of protein expression from the allele was confirmed by Western blots on antibody-stimulated B cells and MEFs. (J:240453)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map3k14 Mutation: 42 strains or lines available

References

• Original: J:240453 Brightbill HD, et al., Conditional Deletion of NF-kappaB-Inducing Kinase (NIK) in Adult Mice Disrupts Mature B Cell Survival and Activation. J Immunol. 2015 Aug 01;195(3):953-64
• All: 1 reference(s)