Babam2^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Babam2^{tm1b(EUCOMM)Wtsi}
• Name: BRISC and BRCA1 A complex member 2; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898381
• Gene: Babam2 Location: Chr5:31855394-32242083 bp, + strand Genetic Position: Chr5, 17.27 cM
• Alliance: Babam2^{tm1b(EUCOMM)Wtsi} page
• IMPC: Babam2 gene page

Mutation origin

• Mutant Cell Line: EPD0065_4_A01
• Germline Transmission: Earliest citation of germline transmission: J:235690
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 31887866 of Chromosome 5 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 3 at position 31888629. The critical exon 3 is thus flanked by loxP sites. Cre expression without flp expression creates a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis and immunofluorescence confirmed the absence of protein expression in fibroblasts. (J:235690)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Babam2 Mutation: 34 strains or lines available

References

• Original: J:235690 Shi W, et al., BRE plays an essential role in preventing replicative and DNA damage-induced premature senescence. Sci Rep. 2016;6:23506
• All: 1 reference(s)