Tmem70^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem70^{tm1b(KOMP)Wtsi}
• Name: transmembrane protein 70; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5897275
• Gene: Tmem70 Location: Chr1:16735431-16748499 bp, + strand Genetic Position: Chr1, 5.1 cM, cytoband A3
• Alliance: Tmem70^{tm1b(KOMP)Wtsi} page
• IMPC: Tmem70 gene page

Mutation origin

• Mutant Cell Line: EPD0288_2_F12
• Germline Transmission: Earliest citation of germline transmission: J:238642
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 16737405 of Chromosome 1 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 16738239. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked exon 2. (J:238642)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem70 Mutation: 17 strains or lines available

References

• Original: J:238642 Vrbacky M, et al., Knockout of Tmem70 alters biogenesis of ATP synthase and leads to embryonal lethality in mice. Hum Mol Genet. 2016 Nov 01;25(21):4674-4685
• All: 1 reference(s)