Slc44a2^tm1.2Tec
Targeted Allele Detail

Summary

• Symbol: Slc44a2^tm1.2Tec
• Name: solute carrier family 44, member 2; targeted mutation 1.2, Thomas E Carey
• MGI ID: MGI:5829833
• Synonyms: Slc44a2^delta
• Gene: Slc44a2 Location: Chr9:21232015-21266324 bp, + strand Genetic Position: Chr9, 7.77 cM
• Alliance: Slc44a2^tm1.2Tec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239440
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector containing an FRT-flanked PGK-Neo selection cassette followed by a loxP site inserted upstream of exon 3 and another loxP site was inserted downstream of exon 10. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 3 through 10 floxed. Germ line, cre mediated recombination removed exons 3-10, which contain the first and largest extracellular loop of the protein. Immunoprecipitation of lung and kidney tissues confirmed that no protein was expressed in mutant mice. (J:239440)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc44a2 Mutation: 48 strains or lines available

References

• Original: J:239440 Kommareddi P, et al., Hair Cell Loss, Spiral Ganglion Degeneration, and Progressive Sensorineural Hearing Loss in Mice with Targeted Deletion of Slc44a2/Ctl2. J Assoc Res Otolaryngol. 2015 Dec;16(6):695-712
• All: 3 reference(s)