Hlcs^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hlcs^em1(IMPC)J
• Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase); endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5817955
• Synonyms: Hlcs^-, Hlcs^em1J
• Gene: Hlcs Location: Chr16:93929741-94114430 bp, - strand Genetic Position: Chr16, 55.12 cM, cytoband C4
• Alliance: Hlcs^em1(IMPC)J page
• IMPC: Hlcs gene page

Hlcs^em1(IMPC)J/Hlcs^em1(IMPC)J mice exhibit embryonic lethality. Embryos are recovered at E7.5 but arrest prior to gastrulation and no embryos are seen at E9.5. Embryos are smaller and form a rudimentary egg cylinder but no primitive streak or gastrulation are seen at E7.5.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory using Cas9 and guides with spacer sequences CATGCCCTTTAAGGCTTGAG, GGAAGTGCGTTCTAATCTGC, GGTGTTGGTATAGTGCTTTT, and GTGTGCAGTCAACAGGTGCG that targeted exon(s) ENSMUSE00000516790.4. This resulted in deletion(s) of 3 bp (location: Chr16:94068168-94068170; GRCm39), 1039 bp (location: Chr16:94068202-94069240; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hlcs Mutation: 43 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 6 reference(s)