Prdx6^tm2.1Abf
Targeted Allele Detail

Summary

• Symbol: Prdx6^tm2.1Abf
• Name: peroxiredoxin 6; targeted mutation 2.1, Aron B Fisher
• MGI ID: MGI:5812685
• Synonyms: Prdx6 S32T
• Gene: Prdx6 Location: Chr1:161067682-161078780 bp, - strand Genetic Position: Chr1, 69.75 cM
• Alliance: Prdx6^tm2.1Abf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237406
• Parent Cell Line: EAP6 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Single point mutation
• Mutation details: The targeting vector is designed to insert, by site-directed mutagenesis, a T(CG) to A(CG) point mutation followed by a FRT-flanked neomycin cassette 290 bp downstream of exon 1. The mutation results in an alteration of the corresponding amino acid from serine to threonine (S32T) at codon 32. Flp-mediated recombination removed the FRT-flanked neo cassette. The mutation does not affect phospholipase A2 activity, but does result in impaired trafficking of PRDX6 to the into lamellar bodies and lysosomes of the lung epithelium. (J:237406)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prdx6 Mutation: 50 strains or lines available

References

• Original: J:237406 Sorokina EM, et al., Mutation of Serine 32 to Threonine in Peroxiredoxin 6 Preserves Its Structure and Enzymatic Function but Abolishes Its Trafficking to Lamellar Bodies. J Biol Chem. 2016 Apr 22;291(17):9268-80
• All: 1 reference(s)