Igs2^{tm1(CAG-Myc*T58A/luc)Wrey}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(CAG-Myc*T58A/luc)Wrey}
• Name: intergenic site 2; targeted mutation 1, Robert Wechsler-Reya
• MGI ID: MGI:5812580
• Synonyms: H11^{lox-STOP-lox -MycT58A}, Igs2^{tm1(CAG-Myc*T58A/luc)Olvr}, LSL-Myc^T58A
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(CAG-Myc*T58A/luc)Wrey} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239378
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: STOCK Igs2^tm3.1Luo/Luo

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Igs2^{tm1(CAG-Myc*T58A/luc)Wrey} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Myc (MGI:97250)	contains a T58A substitution

• Mutation details: The construct is designed to contain (from 5'to 3') an attB site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked stop cassette (LSL), a myelocytomatosis oncogene (Myc) cDNA with a T58A mutation fused to an internal ribosome entry site (IRES) followed by a luciferase gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and a polyadenylation sequence. This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice on a FVB/N congenic background. TARGATT mice carry the H11 allele ( Igs2^tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. One founder mouse carried the correctly targeted transgenic insertion of the construct into the first and second attP sites of the locus. (J:239378)

Tumor Data

List all tumor models in MMHCdb carrying Igs2^{tm1(CAG-Myc*T58A/luc)Wrey}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Igs2^tm3.1Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 ES cells on a mixed background with 129S1/SvJ, 129X1/SvJ, C57BL/6J, DBA/2J, and FVB/N.

References

• Original: J:239378 Mollaoglu G, et al., MYC Drives Progression of Small Cell Lung Cancer to a Variant Neuroendocrine Subtype with Vulnerability to Aurora Kinase Inhibition. Cancer Cell. 2017 Feb 13;31(2):270-285
• All: 17 reference(s)