Olfm4tm2.1(EGFP/cre/ERT2)Cle
Targeted Allele Detail
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Symbol: |
Olfm4tm2.1(EGFP/cre/ERT2)Cle |
Name: |
olfactomedin 4; targeted mutation 2.1, Hans Clevers |
MGI ID: |
MGI:5805245 |
Synonyms: |
Olfm4-IRES-eGFPCreERT2 |
Gene: |
Olfm4 Location: Chr14:80237742-80260581 bp, + strand Genetic Position: Chr14, 42.78 cM
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Alliance: |
Olfm4tm2.1(EGFP/cre/ERT2)Cle page
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Germline Transmission: |
Earliest citation of germline transmission:
J:232454
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Parent Cell Line: |
IB10/E14IB10 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Inducible, Recombinase, Reporter) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Olfm4tm2.1(EGFP/cre/ERT2)Cle expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector was designed to insert an eGFPCreERT2 (GCE) fusion protein coding sequence and loxp-flanked neo selection cassette into just after the stop codon of the last exon of Olfm4 gene, making use of the endogenous poly A signal. Positive clones were injected into blastocysts to establish the mouse line. The neomycin selection cassette was excised in vivo by crossing the mice with the PGK-Cre mouse strain.
(J:232454)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Olfm4
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Olfm4 Mutation: |
29 strains or lines available
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Original: |
J:232454 Schuijers J, et al., Robust cre-mediated recombination in small intestinal stem cells utilizing the olfm4 locus. Stem Cell Reports. 2014 Aug 12;3(2):234-41 |
All: |
6 reference(s) |
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