Phlpp2^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Phlpp2^{tm1d(EUCOMM)Hmgu}
• Name: PH domain and leucine rich repeat protein phosphatase 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5795604
• Gene: Phlpp2 Location: Chr8:110595174-110671303 bp, + strand Genetic Position: Chr8, 57.33 cM
• Alliance: Phlpp2^{tm1d(EUCOMM)Hmgu} page
• IMPC: Phlpp2 gene page

Mutation origin

• Mutant Cell Line: HEPD0619_1_C08
• Germline Transmission: Earliest citation of germline transmission: J:231241
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 110630450 of Chromosome 8 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 110631337. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:231241)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phlpp2 Mutation: 60 strains or lines available

References

• Original: J:231241 Wen YA, et al., Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis. Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2013-23
• All: 2 reference(s)