P2ry2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: P2ry2^{tm1c(EUCOMM)Hmgu}
• Name: purinergic receptor P2Y, G-protein coupled 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5792951
• Synonyms: P2ry2^fl
• Gene: P2ry2 Location: Chr7:100645775-100661260 bp, - strand Genetic Position: Chr7, 54.47 cM, cytoband F1
• Alliance: P2ry2^{tm1c(EUCOMM)Hmgu} page
• IMPC: P2ry2 gene page

Mutation origin

• Mutant Cell Line: HEPD0557_7_B07
• Germline Transmission: Earliest citation of germline transmission: J:224523
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 100645547 of Chromosome 7 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 100648791. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:224523)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P2ry2 Mutation: 16 strains or lines available

References

• Original: J:224523 Wang S, et al., P2Y2 and Gq/G11 control blood pressure by mediating endothelial mechanotransduction. J Clin Invest. 2015 Aug 3;125(8):3077-86
• All: 3 reference(s)