Or8a1^{tm39(Gnas*)Mom}
Targeted Allele Detail

Summary

• Symbol: Or8a1^{tm39(Gnas*)Mom}
• Name: olfactory receptor family 8 subfamily A member 1; targeted mutation 39, Peter Mombearts
• MGI ID: MGI:5784884
• Synonyms: M71(RDY)-caGs-GFP
• Gene: Or8a1 Location: Chr9:37641273-37642357 bp, - strand Genetic Position: Chr9, 20.8 cM
• Alliance: Or8a1^{tm39(Gnas*)Mom} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233447
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Constitutively active, Hypomorph, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Or8a1^{tm39(Gnas*)Mom} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gnas (MGI:95777)	with two amino acid substitutions (D121R;R122D)

• Mutation details: Two amino acid substitutions (D121R;R122D) were made to change the highly conserved DRY motif to RDY to diminish G-protein signalling, and an internal ribosome entry site followed by a constitutively active Gnas coding sequence (due to a Q227L sequence change) was inserted just downstream of the endogenous coding sequence to give bicistronic expression. (J:233447)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Or8a1 Mutation: 69 strains or lines available

References

• Original: J:233447 Movahedi K, et al., Odorant receptors can mediate axonal identity and gene choice via cAMP-independent mechanisms. Open Biol. 2016 Jul;6(7)
• All: 1 reference(s)