Dlg4^tm1.2Hnz
Targeted Allele Detail

Summary

• Symbol: Dlg4^tm1.2Hnz
• Name: discs large MAGUK scaffold protein 4; targeted mutation 1.2, Haining Zhong
• MGI ID: MGI:5770125
• Synonyms: PSD-95-CreNABLED
• Gene: Dlg4 Location: Chr11:69908029-69938107 bp, + strand Genetic Position: Chr11, 42.96 cM, cytoband B4
• Alliance: Dlg4^tm1.2Hnz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218842
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The last two exons of the gene (exons 19 and 20), including the 3' UTR, were duplicated and inserted downstream of the polyadenylation signal and a neomycin selection cassette flanked by FRT sites. Two loxP sites were inserted to flank the native exons 19 and 20 as well as a neomycin stop cassette. The sequence for mVenus was fused in-frame to the end of the coding sequence in exon 20. The original mutation was generated by homologous recombination in embryonic stem (ES) cells. The FRT-flanked neomycin cassette was subsequently excised via a cross to a germline FLP-expressing strain, yielding Dlg4^tm1.1Hnz. Mice bearing this allele were bred with germline Cre-expressing mice to excise the floxed exons 19 and 20 and enable expression of mVenus-labeled, functional PSD95 (DLG4) synaptic protein.

  DLG4 expression levels are slightly lower in mutant than in wild-type brains. Strong mVenus fluorescence can be detected throughout many brain regions, including hippocampus, neocortex, striatum and thalamus. Relatively little fluorescence is observed in the cerebellum. At higher magnification under a two-photon microscope, dense green fluorescent puncta are found throughout the neuropil of the neocortex, striatum and hippocampus. Label is present in most dendritic spines and exhibits minimal baseline trafficking. This mutation enables the in vivo visualization and unambiguous identification of excitatory shaft synapses in aspiny interneurons. (J:218842)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dlg4 Mutation: 42 strains or lines available

Notes

ES cell line = G1 (129S6/SvEvTac x C57BL/6J)F1.

References

• Original: J:218842 Fortin DA, et al., Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins. J Neurosci. 2014 Dec 10;34(50):16698-712
• All: 5 reference(s)