Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Dlg4tm1.1Hnz
Name:	discs large MAGUK scaffold protein 4; targeted mutation 1, Haining Zhong
MGI ID:	MGI:5588003
Synonyms:	PSD-95-ENABLED
Gene:	Dlg4  Location: Chr11:69908029-69938107 bp, + strand  Genetic Position: Chr11, 42.96 cM, cytoband B4
Alliance:	Dlg4tm1.1Hnz page

Germline Transmission:	Earliest citation of germline transmission: J:218842
Parent Cell Line:	Other (see notes) (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6J)F1

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The last two exons of the gene (exons 19 and 20), including the 3' UTR, were duplicated and inserted downstream of the polyadenylation signal and a neomycin selection cassette flanked by FRT sites. Two loxP sites were inserted to flank the native exons 19 and 20 as well as a neomycin stop cassette. The sequence for mVenus was fused in frame to the end of the coding sequence in exon 20. The FRT-flanked neomycin cassette was excised through crosses with a germline flp strain. (J:218842)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dlg4 Mutation:	42 strains or lines available

Original:	J:218842 Fortin DA, et al., Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins. J Neurosci. 2014 Dec 10;34(50):16698-712
All:	2 reference(s)