Sdcbp^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sdcbp^{tm1c(KOMP)Wtsi}
• Name: syndecan binding protein; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5766222
• Synonyms: Syntenin-floxed
• Gene: Sdcbp Location: Chr4:6365654-6396122 bp, + strand Genetic Position: Chr4, 2.91 cM, cytoband A1
• Alliance: Sdcbp^{tm1c(KOMP)Wtsi} page
• IMPC: Sdcbp gene page

Mutation origin

• Mutant Cell Line: EPD0019_2_A01
• Germline Transmission: Earliest citation of germline transmission: J:222712
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_st0
• Mutation details: The L1L2_st0 cassette was inserted at position 6378514 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 3 at position 6379337. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in the brain. (J:222712)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdcbp Mutation: 27 strains or lines available

References

• Original: J:222712 Sugiura H, et al., Rheb activation disrupts spine synapse formation through accumulation of syntenin in tuberous sclerosis complex. Nat Commun. 2015;6:6842
• All: 2 reference(s)