Col1a1^{tm1(tetO-U2AF1*S34F)Mjwa}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-U2AF1*S34F)Mjwa}
• Name: collagen, type I, alpha 1; targeted mutation 1, Matthew J Walter
• MGI ID: MGI:5752388
• Synonyms: U2AF1(S34F)
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-U2AF1*S34F)Mjwa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221403
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutations: Insertion, Nucleotide substitutions
• Col1a1^{tm1(tetO-U2AF1*S34F)Mjwa} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  U2AF1 (7307)

  U2af1

  (MGI:98884)

• Mutation details: RMCE on KH2 ES cells introduced into the 3' UTR a tetracycline responsive element driving inducible expression of a human U2AF1 cDNA with an S34F mutation. (J:221403)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:221403 Shirai CL, et al., Mutant U2AF1 Expression Alters Hematopoiesis and Pre-mRNA Splicing In Vivo. Cancer Cell. 2015 May 11;27(5):631-43
• All: 4 reference(s)