Trpm7^tm1.1Mkma
Targeted Allele Detail

Summary

• Symbol: Trpm7^tm1.1Mkma
• Name: transient receptor potential cation channel, subfamily M, member 7; targeted mutation 1.1, Masayuki Matsushita
• MGI ID: MGI:5707246
• Synonyms: TRPM7 K1646R, TRPM7^R
• Gene: Trpm7 Location: Chr2:126633485-126718150 bp, - strand Genetic Position: Chr2, 61.76 cM, cytoband F2
• Alliance: Trpm7^tm1.1Mkma page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221252
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

Allele Type: Targeted Mutations: Insertion, Single point mutation Mutation details: Exon 33 was replaced with a floxed neomycin resistance cassette and a modified exon in which a nucleotide substitution (A to G) results in the amino acid substitution of arginine for lysine at position 1646 (K1646R). Cre-mediated recombination removed the selection cassette. Kinase assays showed that native kinase activity was abolished in mouse embryonic fibroblasts and peritoneal macrophages derived from homozygous mutant mice, whereas protein levels remained unchanged relative to wild-type controls. (J:221252)

Generation of the Trpm7^tm1.1Mkma allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trpm7 Mutation: 98 strains or lines available

References

• Original: J:221252 Kaitsuka T, et al., Inactivation of TRPM7 kinase activity does not impair its channel function in mice. Sci Rep. 2014;4:5718
• All: 11 reference(s)