Lck^tm3.1Mal
Targeted Allele Detail

Summary

• Symbol: Lck^tm3.1Mal
• Name: lck proto-oncogene, Src family tyrosine kinase; targeted mutation 3.1, Bernard Malissen
• MGI ID: MGI:5706925
• Synonyms: Lck^S59A
• Gene: Lck Location: Chr4:129442142-129467415 bp, - strand Genetic Position: Chr4, 63.26 cM, cytoband distal
• Alliance: Lck^tm3.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217866
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: A p.S59A mutation (TCC to GCC) was created in exon 2, and a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site were inserted into intron 8 via homologous recombination. Cre-mediated self-excision in the male germline removed the cre+neo cassette. The mutation results in altered kinase activity. (J:217866)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lck Mutation: 96 strains or lines available

References

• Original: J:217866 Paster W, et al., A THEMIS:SHP1 complex promotes T-cell survival. EMBO J. 2015 Feb 3;34(3):393-409
• All: 2 reference(s)