Tg(ITGAM-cre/ERT2,U6-RNAi:Cacna1b)17Ttan
Transgene Detail

Summary

• Symbol: Tg(ITGAM-cre/ERT2,U6-RNAi:Cacna1b)17Ttan
• Name: transgene insertion 17, Tsutomu Tanabe
• MGI ID: MGI:5705582
• Synonyms: Cav2.2KD, Tg(Itgam-cre/ERT2,RNU6-RNAi:Cacna1b)17Ttan
• Transgene: Tg(ITGAM-cre/ERT2,U6-RNAi:Cacna1b)17Ttan Location: unknown
• Alliance: Tg(ITGAM-cre/ERT2,U6-RNAi:Cacna1b)17Ttan page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inducible, Knockdown, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(ITGAM-cre/ERT2,U6-RNAi:Cacna1b)17Ttan involves 1 genes/genome features (Cacna1b) View all
• Tg(ITGAM-cre/ERT2,U6-RNAi:Cacna1b)17Ttan expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  ITGAM (3684)

  Itgam

  (MGI:96607)

• Mutation details: The transgene is composed of the human RNU6 promoter disrupted by a floxed neomycin resistance cassette, short-hairpin RNA targeting Cacna1b, the human GH sequence, and the human promoter driving expression of an inducible cre recombinase gene (cre/ERT2) in microglial/macrophage cells. Five founders were generated and line 17 was selected as the representative line. (J:221994)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	ITGAM (human) Summary of all recombinase alleles driven by ITGAM. Alliance human ITGAM gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:221994 Saegusa H, et al., N-type voltage-dependent Ca2+ channel in non-excitable microglial cells in mice is involved in the pathophysiology of neuropathic pain. Biochem Biophys Res Commun. 2014 Jul 18;450(1):142-7
• All: 3 reference(s)