Tsc22d3^tm1.2Hum
Targeted Allele Detail

Summary

• Symbol: Tsc22d3^tm1.2Hum
• Name: TSC22 domain family, member 3; targeted mutation 1.2, Edith Hummler
• MGI ID: MGI:5698178
• Synonyms: Tsc22d3-2^delta
• Gene: Tsc22d3 Location: ChrX:139440277-139501257 bp, - strand Genetic Position: ChrX, 61.35 cM, cytoband F1
• Alliance: Tsc22d3^tm1.2Hum page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223992
• Parent Cell Line: 2A (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An Frt-flanked neomycin selection cassette followed by one loxP site was inserted 3' to the polyadenylation sites of the gene. A second loxP site was inserted 5' to exon 3. Exons 5 and 6 are common exons present in all three isoforms and are reffered as exon 3-4 in J:273574 for similar construct used to create an allele Tsc22d3. The loxP flanked sequences, including exons 3-6 and the Frt-flanked neomycin selection cassette, were removed via Cre-mediated recombination. (J:223992)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tsc22d3 Mutation: 3 strains or lines available

References

• Original: J:223992 Suarez PE, et al., The glucocorticoid-induced leucine zipper (gilz/Tsc22d3-2) gene locus plays a crucial role in male fertility. Mol Endocrinol. 2012 Jun;26(6):1000-13
• All: 2 reference(s)