Isl1^tm4Sev
Targeted Allele Detail

Summary

• Symbol: Isl1^tm4Sev
• Name: ISL1 transcription factor, LIM/homeodomain; targeted mutation 4, Sylvia M Evans
• MGI ID: MGI:5691395
• Synonyms: Isl1^fl:Neo, Isl1 hypo
• Gene: Isl1 Location: Chr13:116434817-116446225 bp, - strand Genetic Position: Chr13, 64.87 cM, cytoband D2.3
• Alliance: Isl1^tm4Sev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152175
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: Exon 4, which encodes the homeodomain of the protein product, was floxed by the insertion of a loxP site on one end and a loxP site and frt-flanked neo cassette on the other end. This allele was used to generate Isl1^tm2Sev and Isl1^tm2.1Sev. Immunohistochemistry confirmed reduced protein expression in the spinal cord. (J:152175)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Isl1 Mutation: 33 strains or lines available

References

• Original: J:152175 Song MR, et al., Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity. Development. 2009 Sep;136(17):2923-32
• All: 7 reference(s)