Igs2^{tm1.1(CAG-cas9*)Mmw}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1.1(CAG-cas9*)Mmw}
• Name: intergenic site 2; targeted mutation 1.1, Monte Winslow
• MGI ID: MGI:5645518
• Synonyms: H11^Cas9
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1.1(CAG-cas9*)Mmw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222466
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Endonuclease)
• Mutation: Insertion
• Mutation details: The targeting construct contains a full-length attB site, the CMV enhancer/chicken beta-actin core promoter (CAGGS), a loxP-STOP(3xSV40 polyA)- loxP cassette (LSL), a 3xFLAG sequence, a nuclear localization signal (NLS), a human codon-optimized S. pyogenes cas9 gene (hSpCas9), a second NLS and the SV40 polyA signal. The embryonic stem cells used were previously targeted with the H11 allele ( Igs2^tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. Cre-mediated recombination removed the LSL cassette. (J:222466)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:222466 Chiou SH, et al., Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015 Jul 15;29(14):1576-85
• All: 27 reference(s)