Nras^tm1.1Nesh
Targeted Allele Detail

Summary

• Symbol: Nras^tm1.1Nesh
• Name: neuroblastoma ras oncogene; targeted mutation 1.1, Norman E Sharpless
• MGI ID: MGI:5645358
• Synonyms: LSL-Nras^Q61R, LSL-N-Ras^Q61R
• Gene: Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Nras^tm1.1Nesh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220627
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Single point mutation
• Mutation details: The 5' untranslated region (5'-UTR) of the gene is interrupted 1,046 base pairs downstream of the transcription start site by a splice acceptor followed by a 3XSTOP transcriptional/translational termination sequence and a single frt site (remaining after excision of a selection cassette from Nras^tm1Nesh by FLP recombinase). A single point mutation in exon 2 has replaced a nucleotide triplet encoding glutamine at amino acid position 61 with an arginine codon (Q61R); this represents the predominant NRAS mutation observed in human melanomas. (J:220627)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Nras Mutation: 44 strains or lines available

References

• Original: J:220627 Burd CE, et al., Mutation-specific RAS oncogenicity explains NRAS codon 61 selection in melanoma. Cancer Discov. 2014 Dec;4(12):1418-29
• All: 11 reference(s)