Nrastm1Nesh
Targeted Allele Detail
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| Symbol: |
Nrastm1Nesh |
| Name: |
neuroblastoma ras oncogene; targeted mutation 1, Norman E Sharpless |
| MGI ID: |
MGI:5645352 |
| Synonyms: |
Q61R+F |
| Gene: |
Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
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| Alliance: |
Nrastm1Nesh page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:220627
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| Parent Cell Line: |
E14 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Conditional ready) |
| Mutations: |
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Insertion, Single point mutation
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Mutation details: A splice acceptor sequence, a 3XSTOP transcriptional/translational termination sequence and a frt site-flanked neomycin resistance cassette (neor), the entire sequence flanked by loxP sites, was inserted into the 5' untranslated region (5'-UTR) 1,046 base pairs downstream of the transcription start site. A single point mutation was introduced into exon 2 that replaces a nucleotide triplet encoding glutamine at amino acid position 61 with an arginine codon (Q61R); this represents the predominant NRAS mutation observed in human melanomas.
(J:220627)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Nras Mutation: |
42 strains or lines available
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| Original: |
J:220627 Burd CE, et al., Mutation-specific RAS oncogenicity explains NRAS codon 61 selection in melanoma. Cancer Discov. 2014 Dec;4(12):1418-29 |
| All: |
1 reference(s) |
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Analysis Tools
