Orc6^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Orc6^em1(IMPC)J
• Name: origin recognition complex, subunit 6; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5638892
• Synonyms: Orc6^-, Orc6^em1J
• Gene: Orc6 Location: Chr8:86026261-86034907 bp, + strand Genetic Position: Chr8, 41.61 cM, cytoband C3
• Alliance: Orc6^em1(IMPC)J page
• IMPC: Orc6 gene page

Orc6^em1(IMPC)J/Orc6^em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E3.5 but not at E7.5. Embryos at E3.5 appear as dying morulae. Mutants fail to hatch from the zona pellucida with apparent cell death and never reach blastocyst stage in culture.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory using Cas9 and guides with spacer sequences CATCTGTTCAGCAGTAAATG and TACTAAACCACTTTATTCGC that targeted exon(s) ENSMUSE00000212010.4. This resulted in deletion(s) of 435 bp (location: Chr8:86031606-86032040; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Orc6 Mutation: 25 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 5 reference(s)