Kras^tm1.1Hbji
Targeted Allele Detail

Summary

• Symbol: Kras^tm1.1Hbji
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 1.1, Hongbin Ji
• MGI ID: MGI:5638622
• Synonyms: LSL-ER-K-ras^G12D
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm1.1Hbji page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220921
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Conditional ready) Mutation: Insertion Mutation details: A floxed STOP cassette was inserted upstream of a modified exon 1 in which an Esr1 cDNA is fused to the coding sequence with the nucleotide susbtitution that results in the amino acid substitution of glycine with aspartic acid at position 12 (p.G12D). Flp-mediated recombination removed an FRT site flanked neomycin resistance cassette inserted downstream of the modified exon 1. (J:220921)

Generation of the Kras^tm1.1Hbji allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 76 strains or lines available

References

• Original: J:220921 Wang Z, et al., Temporal dissection of K-ras(G12D) mutant in vitro and in vivo using a regulatable K-ras(G12D) mouse allele. PLoS One. 2012;7(5):e37308
• All: 1 reference(s)