Col1a1^{tm12(tetO-GFP/RNAi:Pten)Slowe}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm12(tetO-GFP/RNAi:Pten)Slowe}
• Name: collagen, type I, alpha 1; targeted mutation 12, Scott Lowe
• MGI ID: MGI:5638189
• Synonyms: shPten
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm12(tetO-GFP/RNAi:Pten)Slowe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212919
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm12(tetO-GFP/RNAi:Pten)Slowe} involves 1 genes/genome features (Pten) View all
• Mutation details: Recombinase mediated cassette exchange (RMCE) on KH2 cells, which contain Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and an FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 gene, replaced the FRT-flanked neo cassette with a cassette that contains the tetracycline promoter, an EGFP gene and a short hairpin sequence targeting Pten (shPten.1522). (J:212919)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:212919 Miething C, et al., PTEN action in leukaemia dictated by the tissue microenvironment. Nature. 2014 Jun 19;510(7505):402-6
• All: 1 reference(s)